Protein eletropherogram of hypopharyngeal glands of Apis mellifera L. bees submitted to royal jelly production

Authors

  • Hélio Carlos Rocha Universidade de Passo Fundo, Faculdade de Agronomia e Medicina Veterinária, Passo Fundo, RS
  • Paulo Roberto Rodrigues Ramos Universidade Estadual Paulista, Instituto de Biociências, Departamento de Biofísica, Botucatu, SP
  • Silvia Regina Cunha Funari Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Produção e Exploração Animal, Botucatu, SP

Keywords:

hypopharyngeal gland, honeybee (Apis mellifera L.), pollen, SDS-PAGE, electrophoresis

Abstract

The objective of this research was study the protein electropherogram of hypopharyngeal glands and the royal jelly through eletrophorese in polyacrylamide gel of Apis mellifera L. The samples of hypopharyngeal glands were obtained from 18 beehives of africanized bees and its hybrids, italian and carniolas, submitted to royal jelly production, with or without access to 20% pollen protein. The treatments were: recently emerged, 6, 12, 18 and 24 days-old, with six replication. After the extraction, the glands were conserved in a diluted Ringer solution. The protein dosages were determined in spectrofotometer by the biurete biochemical method. The protein content of each sample was estimated by the absorbance at 540 nm. The PhastSystem and 8-25% gradient mini polyacrylamide gels were used to SDS-PAGE electrophoresis analysis. 500 ng protein sample were loaded in each well and after electrophoresis, the gels were stained with silver according to the manufacturer. In recently emerged adult bees, the electrophoretic pattern of hypopharyngeal glands was characterized by proteins of low, medium and high molecular weight, distributed in 18 bands. As the age progresses, proteins with molecular weight ranging from 67 to 76 Kd, started to prevail. Showing identical patterny to the royal jelly. Differences between treatments were observed at 5% of probability.

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Published

2013-11-29

Issue

Section

GENETIC

How to Cite

Protein eletropherogram of hypopharyngeal glands of Apis mellifera L. bees submitted to royal jelly production. (2013). Bulletin of Animal Husbandry, 60(1), 39-46. http://bia.iz.sp.gov.br/index.php/bia/article/view/1352

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