Resitence profiles from Staphylococcus aureus originating in milk from cows with subclinical mastitis

Authors

  • Luiz Francisco Zafalon Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Bovinos de Leite, Nova Odessa, SP
  • Antônio Nader Filho Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, SP
  • José Victor de Oliveira Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Polo Regional de Desenvolvimento Tecnológico dos Agronegócios da Alta Mogiana, Colina, SP
  • Flávio Dutra de Resende Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Polo Regional de Desenvolvimento Tecnológico dos Agronegócios da Alta Mogiana, Colina, SP

Keywords:

antibiotics, milk, resistance, Staphylococcus aureus

Abstract

Staphylococus aureus is considered the most important infectious agent of bovine mastitis. Milk production is reduced and the ill mammary quarters are the most important reservoirs of the pathogen. The objective of this study was to verify the resistance profiles of S. aureus isolated from 66 quarters with bovine mastitis to ampicillin (10 μg), clindamycin (2 μg ), erythromycin (15 μg), gentamicin (30 μg), oxacillin (1 μg), penicillin (10 UI), sulfa plus trimethoprim (25 μg) and tetracycline (30 μg). The isolates were classified in groups: €œA€, resistant to ampicillin and/or penicillin; €œB€, resistant to ampicillin and/or penicillin and/or oxacillin; €œC€, resistant to sulfa plus trimethoprim; €œD€, sensitivity to all antimicrobials tested. Among 66 isolates, 87.9% showed resistance to b-lactams and 40.9% were resistant to oxacillin. Among isolates of mammary quarters with new cases of mastitis, four classified in the group €œA€ changed to the group €œB€, one classified in the group €œA€ changed to the group €œD€, one of the group €œB€ changed to the group €œA€, while three isolates continued in the same group, two in the group €œA€ and other in the group €œD€. The S. aureus isolates showed higher resistance to b-lactams antimicrobials, as penicillin, ampicillin and oxacillin (77.3%, 68.2%, 40.9%, respectively), while the higher sensitivities registers were to gentamicin, sulfa plus trimethoprim, clindamycin and erythromycin (97.0%, 96.3%, 95.6% and 94.4%, respectively).

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Published

2013-11-27

Issue

Section

ANIMAL REPRODUCTION

How to Cite

Resitence profiles from Staphylococcus aureus originating in milk from cows with subclinical mastitis. (2013). Bulletin of Animal Husbandry, 62(3), 229-234. http://bia.iz.sp.gov.br/index.php/bia/article/view/1301

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