Use of gamma radiation to increase genetic variability in Jaragua grass (Hyparrhenia rufa)

Authors

  • Elizabeth Ann Veasey Instituto de Zootecnia, Seção de Agronomia de Plantas Forrageiras, Nova Odessa, SP
  • Maria José Valarini Instituto de Zootecnia, Seção de Agronomia de Plantas Forrageiras, Nova Odessa, SP
  • Nilza Rocha Mecelis Empresa Brasileira de Pesquisa Agropecuária / Instituto de Zootecnia, Seção de Agronomia de Plantas Forrageiras, Nova Odessa, SP
  • Paulo Bardauil Alcntara Instituto de Zootecnia, Seção de Agronomia de Plantas Forrageiras, Nova Odessa, SP
  • Valquíria De Bem Gomes Alcntara Instituto de Zootecnia, Seção de Agronomia de Plantas Forrageiras, Nova Odessa, SP
  • Augusto Tulman Neto Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Seção de Radiogenética, Piracicaba, SP
  • Marcela Ribeiro Abbado Instituto de Zootecnia, Seção de Agronomia de Plantas Forrageiras, Nova Odessa, SP
  • Marcia Atauri Cardelli Instituto de Zootecnia, Seção de Agronomia de Plantas Forrageiras, Nova Odessa, SP

Keywords:

apomixis, gamma radiation, genetic variability, Hyparrhenia rufa

Abstract

Spontaneous or induced mutations provide important genetic variability, useful to many plant species breeding programs. wit the objective to observe mutagenic effect of gamma radiation in Jaragua grass (Hyparrhenia rufa), seeds were irradiated with the dose of 400 Gy from 60Co source. The M1 generation was evaluated in the field. Different morphological characters were recorded, such as divergent racemes, awnless spikelets, low growth, narrow leaves, and chimerism. Vo variation in flowering time was observed. A preliminary progeny test with M2 off-type and control plants showed variation within progeny mainly for the character divergent racemes, which presented Mendelian segregation. Embryo sac analysis, by the clearing technique with phase contrast microscopy, showed the presence of single and multiple aposporous embryo sacs as well as a lower frequency of meiotic embryo sacs indicating the facultative apomictic breeding system of this species. Further investigation is needed to verify whether irradiation has caused a break in apomixis.

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Published

2014-01-14

Issue

Section

FORAGE CROPS AND PASTURES

How to Cite

Use of gamma radiation to increase genetic variability in Jaragua grass (Hyparrhenia rufa). (2014). Bulletin of Animal Husbandry, 53, 61-64. http://bia.iz.sp.gov.br/index.php/bia/article/view/993

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