Ovarian response of Nelore cows superovulated with FSH after passive immunization against bovine follicular fluid

Authors

  • Rafael Herrera Alvarez Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Genética e Reprodução Animal, Nova Odessa, SP
  • Lia de Alencar Coelho Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Zootecnia, Pirassununga, SP
  • Cyro Ferreira Meirelles Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Departamento de Ciência Animal, Piracicaba, SP

Keywords:

Superovulation, follicular fluid, passive immunization, bovine, embryo transfer

Abstract

The aim of the present study was to evaluate the ovarian response of cows superovulated with reduced dose of FSH following passive immunization against bovine follicular fluid. Eighteen Nelore cows were used in a cross over design with the following treatments: iv injection of 80 ml of ovine anti- bovine follicular fluid (treatment 1) or ovine non immune serum (treatment 2 and 3). Six h later, cows were superovulated with 200 (treatment 1 and 2) or 400 UI (treatment 3) FSH-LH hormone (Pluset, Lab. Serono) administered in decreasing doses during 4 days. On the third day, the animais received a luteolitic dose of cloprostenol (Veteglan, Lab. Serono) and w'ere inseminated on the induced estrus. The ovarian response was evaluated 6 to 8 days after insemination by counting the corpora lutea (CL) with ultrasound scanning and by evaluation of the embryos recovered using standard techniques of cervical embryo collect. The rate of good response to superovulation (>3CL) of the treatments 1 (94.4%) and 3 (83.3%) was higher and statistically different (P<0.05) from treatment 2 (50.0%). Likewise, tlie mean number of embryos recovered from treatments 1 (4.1 ± 0.9) and 3 (5.2 ± 0.9) was higher and statistically different (P<0.05) from treatment 2 (2.1 ± 0.5). From these results, it can be concluded that passive immunization against bovine follicular fluid increase the ovarian sensitivity to exogenous gonadotropin in Nelore cows different (P<0.05) from treatment 2 (50.0%). Likewise, the mean number of embryos recovered from treatments 1 (4.1 ± 0.9) and 3 (5.2 ± 0.9) was higher and statistically different (P<0.05) from treatment 2 (2.1 ± 0.5). From these results, it can be concluded that passive immunization against bovine follicular fluid increase tlie ovarian sensitivity to exogenous gonadotropin in Nelore cows.

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Published

2013-12-04

Issue

Section

ANIMAL REPRODUCTION

How to Cite

Ovarian response of Nelore cows superovulated with FSH after passive immunization against bovine follicular fluid. (2013). Bulletin of Animal Husbandry, 55(2), 161-166. http://bia.iz.sp.gov.br/index.php/bia/article/view/936

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