Effect of several extenders in frozen-thawed buffalo semen submitted to thermo-resistence test

Authors

  • Geraldo Moose Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Genética e Reprodução Animal, Nova Odessa, SP
  • João Batista Pereira de Carvalho Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Pólo Regional de Desenvolvimento Tecnológico dos Agronegócios do Vale do Paraíba, Pindamonhangaba, SP
  • Benedicto do Espírito Santo de Campos Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Genética e Reprodução Animal, Nova Odessa, SP
  • José Ramos Nogueira Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Polo Regional de Desenvolvimento Tecnológico dos Agronegócios do Centro Leste, Ribeirão Preto, SP
  • Roberto Hauck Reichert Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Pólo Regional de Desenvolvimento Tecnológico dos Agronegócios Vale do Ribeira, Unidade de Pesquisa e Desenvolvimento de Registro, Registro, SP

Keywords:

buffalo, freeze-thaw, extenders, semen, thermo-resistance test

Abstract

Samples of frozen semen (N = 139) from four water buffaloes (Jafarabadi and Murrah crossbred), with three years of age, were collected from January to November of 1990, were used. Semen was collected weekly and evaluated by microscope for physical characteristics of turbulence; concentration; motility and vigor. Fresh semen was diluted in solution prepared with seven extenders: yolk-lactose; TRIS-I; TRIS-II; TRIS-III; yolk-cytrate; TES-TRIS and MEN-Eagle medium. Concentrations of glycerol (7%); egg-yolk (20%) and distilled water to complete 100ml were constant for all extenders. The Thermo-Resistance Test (TRT) was used to achieve motility and vigor in frozen-thawed semen at zero and five hours of incubation. There was no difference among extenders (P>0.01) for motility and vigor at zero hour of incubation; but at five hours there were differences (P<0.01) among semen frozen with yolk-lactose; TRIS-I and TES-TRIS. TES-TRIS showed higher motility and vigor than the TRIS-I. Based on these results it is possible to freeze-thaw buffalo semen with these extenders. An appropriate concentration of sugar in the solution could improve motility of thawed buffalo semen.

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Published

2007-03-22

Issue

Section

ANIMAL REPRODUCTION

How to Cite

Effect of several extenders in frozen-thawed buffalo semen submitted to thermo-resistence test. (2007). Bulletin of Animal Husbandry, 64(3), 233-239. http://bia.iz.sp.gov.br/index.php/bia/article/view/1234

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