Techniques used detection and quantification of aflatoxin M1 in milk

Authors

  • Adriana Frizzarin Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Nova Odessa, SP
  • Keila Maria Roncato Duarte Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Genética e Reprodução Animal, Nova Odessa, SP

Keywords:

mycotoxins, AFM1, ELISA, chromatography, HPLC

Abstract

Aflatoxin is a group of toxic substances produced by fungi, mainly Aspergillus flavus and Aspergillus parasiticus. It can be developed in agriculture products such as grains or processed food, when environment conditions of humidity and air humidity are favorable. Aflatoxins can be presented as several forms. In Milk, are called M1 and M2, resulting from aflatoxins B1 and B2 metabolism. Aflatoxin M1 (AFM1) is classified as a possible carcinogen to humans, so the occurrence of aflatoxin M1 in milk of lactating cows is a public health issue, and because of its importance several techniques are used for its detection and quantification. These techniques include the physical-chemical as thin layer chromatography and high performance liquid chromatography and the biological techniques including immunoassays such as RIA and ELISA. This review aimed to present the techniques used to quantify aflatoxins M1 and M2 in milk and dairy products.

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Published

2012-02-11

Issue

Section

ANIMAL QUALITY PRODUCTS

How to Cite

Techniques used detection and quantification of aflatoxin M1 in milk. (2012). Bulletin of Animal Husbandry, 69(2), 165-173. http://bia.iz.sp.gov.br/index.php/bia/article/view/1037

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