Embryos retained in the oviduct as a factor of variation on the final recovery rate after uterus flushing in superovulated cows

Authors

  • Rafael Herrera Alvarez Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Genética e Reprodução Animal, Nova Odessa, SP
  • João Batista Pereira de Carvalho Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Núcleo de Pesquisas Zootécnicas Geraldo José Rodrigues Alckmin, Pindamonhangaba, SP
  • Maria Ines de Aquino Barbosa Carvalho Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Núcleo de Pesquisas Zootécnicas Geraldo José Rodrigues Alckmin, Pindamonhangaba, SP

Keywords:

Bovine, oviduct, embryos, recovery rate

Abstract

Usually, the number of embryos recovered from the uterus of superovulated cows not correspond to the number of corpora lutea (CL) presents on the ovary at time of embryo collection. The aim of this study was to evaluate if embryos retained in the oviduct could explain the low rates of embryo recovery. Fifty two crossbred cows were superovulated in the middle of the estrus cycle (days 8 to 12) with twice daily FSL-LH injections, during 4 days. On the morning of the third day the animals were given with a luteolitic dose of prostaglandin F2a analogue and mated with a bull at the induced estrous. Seven days after mating, the cows were slaughtered and the genital tract recovered for visual inspection of ovarian structures. The uterus was flushed with approximately 100 ml PBS (added of 10% bovine fetal serum), using a Foley catheter and the oviduct was injected (in the sense fimbria to isthmus) with approximately 50 ml PBS, using a needle coupled to a syringe. The embryos were searched and morphologically evaluated (following IETS criteria) under a stereoscopy. At all, 805 CL (mean 15.5±0.9) were counted, with 49 cows showing more than 3 CL. The embryo recovery rate was 81.1% (653 embryos). Thirteen cows (25%) retained one or more embryos into the oviduct, contributing with 4.2% (34 embryos – ten of them viable embryos) from all the embryos. It was concluded that part of the variation of the embryo recovery rate from cows superovuladed with FSH may be due to the embryos retained into the oviduct.

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Published

2013-12-03

Issue

Section

ANIMAL REPRODUCTION

How to Cite

Embryos retained in the oviduct as a factor of variation on the final recovery rate after uterus flushing in superovulated cows. (2013). Bulletin of Animal Husbandry, 56(1), 89-93. http://bia.iz.sp.gov.br/index.php/bia/article/view/924

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