In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control

Autores

  • Ida Rúbia Machado Moulin Universidade Estadual do Norte Fluminense, UENF, Campos dos Goytacazes, RJ, Brazil
  • Isabella da Costa Teixeira Instituto Federal do Espírito Santo, Campus Alegre, Alegre, ES, Brazil
  • Ueldiane Quintiliano Lins Instituto Federal do Espírito Santo, Campus Alegre, Alegre, ES, Brazil
  • Aparecida de Fátima Madella-Oliveira Instituto Federal do Espírito Santo, Campus Alegre, Alegre, ES, Brazil
  • Tercio da Silva de Souza Instituto Federal do Espírito Santo, Campus Alegre, Alegre, ES, Brazil

Palavras-chave:

Alternative control, dairy herds, phytotherapy

Resumo

Bovine mastitis is a complex and multifactorial disease that affects dairy herds, leading to severe economic losses to producers. Given this scenario, there is a need to find alternative methods that are effective and safe for animal and human health. The use of natural compounds derived from plants is an alternative for the treatment of mastitis in dairy cows. We evaluated the in vitro antimicrobial activity of the essential oils (EO) of cinnamon (Cinnamomum sp.), melaleuca (Melaleuca alternifolia) and oregano (Origanum vulgare) against Staphylococcus aureus (ATCC 25923), aiming at future application for the control of bovine mastitis. The tests were performed in triplicate for the fixed concentration of 5 mg (50000µg disc-1) of EO. The evaluation was done by the disk diffusion method, in which trypticase soy broth (TSB) medium was inoculated with the microorganism S. aureus at 108 UFC mL-1 by pouring the medium into sterile Petri dishes with 140 mm diameter. After inoculation, sterile filter paper discs with 6 mm diameter impregnated with 5 mg of each EO were placed on the medium. After 24 h incubation at 37±1 ºC, the inhibition halos around the discs were measured. In the statistical analysis, a completely randomized design was used, in a 2 x 3, factorial scheme consisting of tea tree and eucalyptus essential oils on S. aureus strains, with 3 repetitions. The final results, determined by the arithmetic mean of the inhibition halos (mm), were submitted to analysis of variance (p<0.01) and the SNK test (p<0.05), using PROC GLM. The effect of the 5 mg concentration of oregano EO showed higher sensitivity against melaleuca EO (10.54 mm) and cinnamon EO (14.01 mm), presenting a halo of 19.09 mm. The results demonstrated that oregano essential oil has potential antibacterial activities and showed satisfactory results at a small concentration. Thus, oregano OE showed excellent action against bacterial growth. However, there is a need for further studies to evaluate its potential against standard strains such as ATCC 25923.

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Publicado

30-12-2022

Como Citar

In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control. (2022). Boletim De Indústria Animal, 79. http://bia.iz.sp.gov.br/index.php/bia/article/view/1887

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